| Solution Information | help | |
| Enzyme: | Oxytocin receptor | |
| inhibitor: | BDBM75710 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | Assay Overview: The purpose of this assay is to determine dose response curves for compounds identified as active in a previous set of experiments entitled, "Fluorescence-based primary cell-based high throughput screening assay to identify potentiators of Oxytocin Receptor (OXTR)" (AID 2445), and that confirmed activity in a set of experiments entitled, "Fluorescence-based cell-based high throughput confirmation assay to identify potentiators of Oxytocin Receptor (OXTR)" (AID 434985). In this assay a CHO cell line that stably expresses OTR is incubated with a fluorescent, cell permeable calcium indicator dye (such as Fluo-8), followed by the addition of test compounds and agonist. The dye serves to monitor levels of intracellular calcium in the CHO-OTR cells. As designed, compounds that act as OTR potentiators will increase calcium mobilization, resulting in increased relative fluorescence of the indicator dye, and increased well fluorescence. Compounds are tested in triplicate using | |
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